Recombinant Canine IL6 protein, His-tagged

Cat.No.: IL6-144C
Product Overview: Recombinant Canine IL6(21-207aa) fused with His tag at C-terminal was expressed in Insect cells.
Description: IL6, as known as Interleukin-6, is a phosphorylated and variably glycosylated cytokine. It is secreted by T cells and macrophages to stimulate immune response during infection and after trauma, especially burns or other tissue damage leading to inflammation. Also, this protein can function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise.
Source: Insect cells
Species: Canine
Tag : His
Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Molecular Mass: 22.0kDa (195aa)
18-28kDa (SDS-PAGE under reducing conditions.)
AA Sequence: FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEC VKHTTIHLIL RSLEDFLQFS
LRAVRIMLEH HHHHH
Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity: > 95% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 0.5mg/ml (determined by Bradford assay)
Gene Name: IL6 interleukin 6 [ Canis lupus familiaris ]
Official Symbol: IL6
Synonyms: IL-6; interleukin-6; interleukin 6 (interferon, beta 2)
Gene ID: 403985
mRNA Refseq: NM_001003301
Protein Refseq: NP_001003301
UniProt ID: P41323
Chromosome Location: IL-6
Pathway: AGE-RAGE signaling pathway in diabetic complications, organism-specific biosystem; Antifolate resistance, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem
Function: cytokine activity; interleukin-6 receptor binding; growth factor activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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