Recombinant Cynomolgus Cd274 protein, His-tagged, Biotinylated, Primary Amine Labeling

• Cat.No.: Cd274-6743C
• Product Overview: Recombinant Cynomolgus Cd274 Protein (G7PSE7, 19-238aa), was expressed in HEK293 with C-terminal His tag and primary amine labeling biotinylated conjugate.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 19-238 aa
• Tag: C-His
• Conjugation/Label: Biotinl; Primary Amine Labeling
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin