Active Recombinant Cynomolgus CSF1 protein, His-tagged, Low Endotoxin

• Cat.No.: CSF1-1725C
• Product Overview: Recombinant Cynomolgus CSF1 protein(XP_045221641.1)(33-255 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 33-255 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus M-CSF, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.26 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.