Recombinant Cynomolgus ERBB2 Protein, His-tagged

Cat.No.: ERBB2-1217C
Product Overview: Recombinant cynomolgus ERBB2 (Met1-Thr652) [similar to Macaca fascicularis ERBB2 (EHH58073.1)] was expressed with a polyhistidinetag atthe C-terminus.
Source: HEK293
Species: Cynomolgus
Tag: His
Predicted N Terminal: Thr 23
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus ERBB2-His at 10 μg/ml (100 μl/well) can bind Herceptin, The EC50 of Herceptin is 16.4-38.4 ng/ml.
Molecular Mass: The recombinant cynomolgus ERBB2 comprises 641 amino acids and has a calculated molecular mass of 70.8 KDa. The apparent molecular mass of it is approximately 80-105 KDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: ERBB2 erb-b2 receptor tyrosine kinase 2 [ Macaca fascicularis ]
Official Symbol: ERBB2
Synonyms: erb-b2 receptor tyrosine kinase 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
Gene ID: 102146608
mRNA Refseq:
Protein Refseq:
MIM:
UniProt ID:
Chromosome Location: chromosome: 16
Pathway: Adherens junction, organism-specific biosystem; Focal adhesion, organism-specific biosystem; MicroRNAs in cancer, conserved biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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