Recombinant Cynomolgus FCGR1A protein, His-Avi-tagged, Biotinylated

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Recombinant Cynomolgus FCGR1A protein, His-Avi-tagged, Biotinylated

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Cat.No. : FCGR1A-3818C
Product Overview : Recombinant Cynomolgus FCGR1A Protein (Val11-Pro288) is produced by HEK293 expression system. This protein is fused with a His-Avi tag at the C-terminal. The protein has a calculated MW of 34.8 kDa. The protein migrates as 40-50 kDa under reducing (R) con
Description : Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
Source : HEK293
Species : Cynomolgus
Tag : His-Avi
Predicted N Terminal : Val 11
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized FMC63 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) with a linear range of 0.2-10 ng/mL. Loaded Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) on SA Biosensor, can bind Herceptin with an aff
Molecular Mass : 34.8 kDa
Protein length : Val11-Pro288
Endotoxin : <1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution.
Synonyms : FCGR1A; FCG1; FCGR1; IGFR1; CD64; CD64A; FCRI;
Figure :

Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

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