Recombinant Cynomolgus IGF1R protein, His-tagged

• Cat.No.: IGF1R-391H
• Product Overview: Recombinant Cynomolgus IGF1R protein (Glu31-Asn932), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 104.9 kDa, 81.1 kDa, 23.8 kDa. The protein migrates as 45-50 kDa, 96-115 kDa and 120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 902
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IGF1R
• Official Symbol: IGF1R
• Synonyms: IGF1R; insulin-like growth factor 1 receptor; IGF-I receptor; insulin-like growth factor I receptor; JTK13; IGFIRC
• Gene ID: 708227
• mRNA Refseq: NM_001261352.1
• Protein Refseq: NP_001248281.1
• UniProt ID: I2CWY3

Reviews

07/08/2022

The product's exceptional purity facilitated precise quantification, ensuring data consistency.

06/14/2019

The product consistently delivered robust and dependable results, affirming its efficacy in our experiments.

04/29/2018

The product's consistent performance bolstered the dependability of our research conclusions.

Q&As

In which cellular compartments is IGF1R predominantly localized?

IGF1R is predominantly localized on the cell membrane, where it acts as a receptor for insulin-like growth factors (IGFs) and insulin.

Are there specific downstream signaling pathways activated by IGF1R, and how do they influence cell proliferation and survival?

Downstream signaling pathways activated by IGF1R include the Ras/MAPK pathway and PI3K/Akt pathway, contributing to cell growth and survival.

How does IGF1R interact with insulin-like growth factors (IGFs) and insulin, and what is its role in growth regulation?

IGF1R interacts with IGFs and insulin, triggering downstream signaling pathways such as the PI3K/Akt pathway, influencing cell proliferation and survival.

Are there therapeutic implications or potential targets related to IGF1R in diseases involving growth signaling?

There are potential therapeutic implications related to IGF1R in diseases involving growth signaling, making it a target for drug development.

What diseases or conditions are associated with dysregulation or abnormal expression of IGF1R?

Dysregulation or abnormal expression of IGF1R is associated with conditions such as cancer and developmental disorders.

What is the primary function of Insulin-like growth factor 1 receptor (IGF1R) in cellular processes and growth signaling?

Insulin-like growth factor 1 receptor (IGF1R) functions in cellular processes, primarily playing a role in growth signaling.

How does IGF1R contribute to the regulation of tissue development, particularly in the context of growth and differentiation?

IGF1R contributes to the regulation of tissue development by influencing cell growth, differentiation, and survival.