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Recombinant Cynomolgus IGF1R Protein, His-tagged

Cat.No. : IGF1R-391H
Product Overview : Recombinant Cynomolgus IGF1R Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # G7P9I7-1). Predicted N-terminus: Glu 31, Asp 741. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 104.9 kDa, 81.1 kDa, 23.8 kDa. The protein migrates as 45-50 kDa, 96-115 kDa and 120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Cynomolgus
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Recombinant Cynomolgus IGF1R Protein, His-tagged at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.02-0.3 μg/mL (QC tested).
Molecular Mass : 104.9 kDa
Protein length : Glu 31 - Asn 932
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
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