Recombinant Cynomolgus IL12RB1 protein, His-tagged

• Cat.No.: IL12RB1-671C
• Product Overview: Recombinant Cynomolgus IL12RB1 protein(XP_015296347.1) (Met1-Asp545) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Cynomolgus IL12RB1 consists of 533 amino acids and predicts a molecular mass of 58.98 kDa. It migrates as an approximately 78.33 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Asp545
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >90% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Reviews

04/06/2022

Reliable in co-immunoprecipitation.

10/18/2021

Effective in asthma models.

12/21/2017

great in Western blot analysis.

Q&As

Could you explain the significance of CRISPR/Cas9-mediated gene tagging in tracking IL12RB1 localization?

Gene tagging allows real-time tracking of IL12RB1 subcellular localization.

Can you elaborate on the use of RNA-seq analysis to profile gene expression changes upon IL12RB1 activation?

RNA-seq profiles gene expression changes upon IL12RB1 activation.

What are the applications of ChIP-seq in exploring IL12RB1's role in gene regulation?

ChIP-seq investigates IL12RB1's impact on gene regulation networks.

What methods can be employed to assess IL12RB1 dimerization and its effect on downstream signaling?

Crosslinking and immunoprecipitation assess IL12RB1 dimerization and signaling.

What techniques are suitable for studying IL12RB1's interaction with IL12A and IL23A?

Co-immunoprecipitation and ELISA assay IL12RB1 interactions with cytokines.

How can the role of IL12RB1 in signal transduction be elucidated using knockdown experiments?

Knockdown experiments reveal IL12RB1's contribution to signal transduction.

What are the advantages of using siRNA screening to identify IL12RB1-regulated pathways?

siRNA screening identifies pathways influenced by IL12RB1 expression.

How do co-crystallization studies aid in understanding the molecular details of IL12RB1 interactions?

Co-crystallization offers structural insights into IL12RB1 interactions.

How does flow cytometry provide insights into IL12RB1 expression levels on different cell types?

Flow cytometry quantifies IL12RB1 levels on diverse cell populations.

What experimental approaches validate IL12RB1's involvement in cytokine-mediated immune responses?

Functional assays validate IL12RB1's role in immune responses.