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Recombinant Cynomolgus IL12RB1 protein, His-tagged

Cat.No. : IL12RB1-671C
Product Overview : Recombinant Cynomolgus IL12RB1 protein(XP_015296347.1) (Met1-Asp545) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.
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Source : HEK293
Species : Cynomolgus
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant Cynomolgus IL12RB1 consists of 533 amino acids and predicts a molecular mass of 58.98 kDa. It migrates as an approximately 78.33 kDa band in SDS-PAGE under reducing conditions.
Protein length : Met1-Asp545
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : >90% as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Not For Human Consumption!

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Customer Reviews (3)

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Reviews
04/06/2022

    Reliable in co-immunoprecipitation.

    10/18/2021

      Effective in asthma models.

      12/21/2017

        great in Western blot analysis.

        Q&As (10)

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        Could you explain the significance of CRISPR/Cas9-mediated gene tagging in tracking IL12RB1 localization? 09/18/2021

        Gene tagging allows real-time tracking of IL12RB1 subcellular localization.

        Can you elaborate on the use of RNA-seq analysis to profile gene expression changes upon IL12RB1 activation? 07/02/2021

        RNA-seq profiles gene expression changes upon IL12RB1 activation.

        What are the applications of ChIP-seq in exploring IL12RB1's role in gene regulation? 01/23/2021

        ChIP-seq investigates IL12RB1's impact on gene regulation networks.

        What methods can be employed to assess IL12RB1 dimerization and its effect on downstream signaling? 06/13/2020

        Crosslinking and immunoprecipitation assess IL12RB1 dimerization and signaling.

        What techniques are suitable for studying IL12RB1's interaction with IL12A and IL23A? 02/14/2020

        Co-immunoprecipitation and ELISA assay IL12RB1 interactions with cytokines.

        How can the role of IL12RB1 in signal transduction be elucidated using knockdown experiments? 12/27/2019

        Knockdown experiments reveal IL12RB1's contribution to signal transduction.

        What are the advantages of using siRNA screening to identify IL12RB1-regulated pathways? 02/22/2019

        siRNA screening identifies pathways influenced by IL12RB1 expression.

        How do co-crystallization studies aid in understanding the molecular details of IL12RB1 interactions? 01/02/2019

        Co-crystallization offers structural insights into IL12RB1 interactions.

        How does flow cytometry provide insights into IL12RB1 expression levels on different cell types? 10/26/2018

        Flow cytometry quantifies IL12RB1 levels on diverse cell populations.

        What experimental approaches validate IL12RB1's involvement in cytokine-mediated immune responses? 06/25/2018

        Functional assays validate IL12RB1's role in immune responses.

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