Recombinant Cynomolgus MAPT Protein, His tagged

• Cat.No.: MAPT-02C
• Product Overview: Recombinant Cynomolgus MAPT Protein with His tag was expressed in HEK293.

Specification

• Description: This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Molecular Mass: The protein has a calculated MW of 44 kDa.
• AA Sequence: AEPRQEFDVMEDHAGTYGLGDRKDQ EGYTMLQDQEGDTDAGLKESPLQTP AEDGSEELGSETSDAKSTPTAEAEE AGIGDTPSLEDEAAGHVTQARMVSK SKDGTGSDDKKAKGADGKTKIATPR GAAPPGQKGQANATRIPAKTPPAPK TPPSSGEPPKSGDRSGYSSPGSPGT PGSRSRTPSLPTPPAREPKKVAVVR TPPKSPSSAKSRLQTAPVPMPDLKN VKSKIGSTENLKHQPGGGKVQIINK KLDLSNVQSKCGSKDNIKHVPGGGS VQIVYKPVDLSKVTSKCGSLGNIHH KPGGGQVEVKSEKLDFKDRVQSKIG SLDNITHVPGGGNKKIETHKLTFRE NAKAKTDHGAEIVYKSPVVSGDTSP RHLSNVSSTGSIDMVDSPQLATLAD EVSASLAKQGLHHHHHHHH
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.73 mg/mL by BCA
• Storage Buffer: PBS, pH 7.4

Gene Information

• Gene Name: MAPT microtubule associated protein tau [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: MAPT
• Synonyms: MAPT; microtubule associated protein tau; microtubule-associated protein tau
• Gene ID: 102119414
• mRNA Refseq: XM_005584543
• Protein Refseq: XP_005584600
• UniProt ID: A0A2K5WBI2

Reviews

07/22/2022

Western-blot experiments using MAPT as a target have shown highly reproducible results.

08/08/2020

This stability of MAPT is very good and can maintain its original properties even after multiple uses.

09/15/2019

MAPT's purity and consistency make it a top choice for Western-blot experiments.

Q&As

How to assess the degree of abnormal aggregation of MAPT protein?

The degree of abnormal aggregation of MAMAP proteins can be assessed by a variety of methods, such as immunohistochemistry, immunofluorescence staining, Western blot, etc. These methods can detect and quantify abnormally aggregated MAPT proteins.

What is the relationship between MAPT protein and nerve cytoskeleton?

The MAPT protein is closely related to the nerve cytoskeleton. It is involved in regulating axon formation and maintenance, cytosolic flow, and the normal function of neurons by binding and stabilizing microtubules.

What are the interactions of the MAPT protein with other proteins in the nervous system?

MAPT protein interacts with other proteins in the nervous system, such as α-synuclein, β-kinigin, etc. These interactions may be involved in regulating the structure and function of neurons.

Is there a mechanism that regulates the expression of MAPT protein?

Yes, the expression of MAPT protein is affected by a variety of regulatory mechanisms, including transcriptional regulation, splicing regulation, phosphorylation regulation, etc. These regulatory mechanisms are essential for maintaining normal MAPT protein levels and function.

What are the future directions for MAPT protein research?

Future research directions include further elucidating the mechanism of abnormal aggregation and phosphorylation of MAPT protein, exploring the pathogenesis of genetic diseases associated with MAPT gene, finding therapeutic strategies to intervene in abnormal aggregation of MATT, and developing biomarkers related to MAPT protein for early diagnosis and therapeutic monitoring.

How about the applications of MAPT protein in clinical diagnosis?

The MAPT protein plays an important role in clinical diagnosis. By measuring the level of the MAPT protein in the blood or cerebrospinal fluid, it can help identify different types of neurodegenerative diseases, such as Alzheimer's disease and prefrontal temporal dementia.