Active Recombinant Cynomolgus Monkey IL18BP Protein, His-tagged

• Cat.No.: IL18BP-56C
• Product Overview: Recombinant Cynomolgus Monkey IL18BP (Thr28-Arg207) with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: CHO
• Species: Cynomolgus Monkey
• Tag: His
• Bio-activity: Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.015-0.075 μg/mL
• Molecular Mass: 20 kDa
• Protein length: 28-207
• N-terminal Sequence Analysis: Thr28
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: IL18BP interleukin 18 binding protein [ Homo sapiens (human) ]
• Official Symbol: IL18BP
• Synonyms: IL18BP; interleukin 18 binding protein; FVH; IL18BPa; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa
• Gene ID: 10068
• mRNA Refseq: NM_173044
• Protein Refseq: NP_766632
• MIM: 604113
• UniProt ID: O95998