| Species : |
Human |
| Source : |
NS0 |
| Tag : |
Fc |
| Protein Length : |
Thr29-Gly192 (IL18BP, 164 aa) + IEGRMD linker + Pro100-Lys330 (IgG1 Fc, 231 aa) |
| Description : |
Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. The protein functions as an inhibitor of the proinflammatory cytokine IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced Th1 immune response. This protein is constitutively expressed and secreted in mononuclear cells. Elevated levels are detected in intestinal tissues of Crohn's disease patients. |
| Form : |
Lyophilized |
| Bio-activity : |
Inhibits Recombinant Human IL-18/IL-1F4 induced IFN-gamma secretion in KG-1 human acute myelogenous leukemia cells. ED50: 0.02-0.12 ug/mL in the presence of 40 ng/mL rhIL-18. |
| Molecular Mass : |
Predicted: 44.2 kDa (monomer); Observed: 60-80 kDa (reducing conditions, SDS-PAGE). Exists as disulfide-linked homodimer. |
| AASequence : |
TPVSQTTTAATASVRSTKDPCPSQPPVFPAAKQCPALEVTWPEVEVPLNGTLSLSCVACSRFPNFSILYWLGNGSFIEHLPGRLWEGSTSRERGSTGTQLCKALVLEQLTPALHSTNFSCVLVDPEQVVQRHVVLAQLWAGLRATLPPTQEALPSSHSSPQQIEGRMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| Endotoxin : |
<0.10 EU/ug (LAL method) |
| Purity : |
>95%, determined by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining |
| Applications : |
Bioactivity |
| Storage : |
Lyophilized: -20C to -70C for 12 months from date of receipt. Reconstituted (sterile): 2-8C for 1 month; -20C to -70C for 3 months. Avoid repeated freeze-thaw cycles. |
| Concentration : |
Reconstitute at 100 ug/mL in sterile PBS |
| Reconstitution : |
Reconstitute at 100 ug/mL in sterile PBS. |
| Storage Buffer : |
Lyophilized from 0.2 um filtered solution in PBS (Carrier Free, no BSA) |
