|Product Overview:||Recombinant Cynomolgus / Rhesus Monkey CD47 Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 141 (Accession # NP_001253446.1). Predicted N-terminus: Gln 19. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Species:||Cynomolgus / Rhesus Monkey|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Human SIRP alpha, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus Monkey CD47 Protein, His-tagged with a linear range of 4-63 ng/mL (QC tested).
Immobilized Anti-CD47, Human IgG4 at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus Monkey CD47 Protein, His-tagged with a linear range of 0.2-5 ng/mL (Routinely tested).
Anti-CD47 Mab (Human IgG4) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Recombinant Cynomolgus / Rhesus Monkey CD47 Protein, His-tagged with an affinity constant of 1.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
|Molecular Mass:||15.8 kDa|
|Protein length:||Gln 19 - Glu 141|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|