Recombinant Dog IL12B protein, His & T7-tagged
|Product Overview :||Recombinant Dog IL12B aa. (Ile23~Ser329 (Accession # Q28268)) fused with N-terminal His & T7 tag was produced in E. coli cells.|
- Gene Information
- Related Products
|Source :||E. coli|
|Tag :||His & T7|
|Form :||Freeze-dried powder|
|Molecular Mass :||Predicted Molecular Mass: 38.6kDa|
|Protein length :||Ile23~Ser329 (Accession # Q28268)|
|Endotoxin :||<1.0EU per 1µg (determined by the LAL method)|
|Purity :||> 95%|
|Characteristic :||The isoelectric point is 6.8.|
|Applications :||SDS-PAGE; WB; ELISA; IP|
|Stability :||The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.|
|Storage :||Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.|
|Storage buffer :||Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl|
|Reconstitution :||Reconstitute in sterile PBS, pH7.2-pH7.4|
|Gene Name :||IL12B interleukin 12B [ Canis lupus familiaris (dog) ]|
|Official Symbol :||IL12B|
|Synonyms :||IL12B; interleukin 12B; interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL-12B; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 p40 subunit|
|Gene ID :||403976|
|mRNA Refseq :||NM_001003292.1|
|Protein Refseq :||NP_001003292.1|
|UniProt ID :||Q28268|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
ChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Customer Reviews (3)Write a review
Good for cytokine assays. -
Effective in Th2 cell differentiation. -
High yield in expression. -
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