Recombinant E.coli Mug, His-tagged
Cat.No. : | mug-93E |
Product Overview : | Recombinant E. coli G/U Mismatch-Specific DNA Glycosylase/Mug is produced with our E.coli expression system. The target protein is expressed with sequence (Met1-Arg168) of E.coli Mug. |
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Description : | E. coli Mismatch Uracil DNA Glycosylase (Mug protein), also known as G/U mismatch-specific DNA glycosylase, is an 18 kDa constitutively expressed protein which belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3,N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. Uracil bases in DNA can arise from deamination of cytosine giving rise to increased spontaneous mutations. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells. |
Source : | E. coli |
Species : | E. coli |
Tag : | His |
Form : | Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 2.5mM β-ME, 1mM PMSF, 50% Glycerol, pH 8.0 |
AA Sequence : | MVEDILAPGLRVVFCGINPGLSSAG TGFPFAHPANRFWKVIYQAGFTDRQ LKPQEAQHLLDYRCG VTKLVDRPTVQANEVSKQELHAGGR KLIEKIEDYQPQALAILGKQAYEQG FSQRGAQWGKQTLTI GSTQIWVLPNPSGLSRVSLEKLVEA YRELDQALVVRGRLEHHHHHH |
Purity : | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Storage : | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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