Recombinant Escherichia coli EFP Protein (2-188 aa), His-SUMO-tagged

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Recombinant Escherichia coli EFP Protein (2-188 aa), His-SUMO-tagged

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Cat.No. : EFP-1865E
Product Overview : Recombinant Escherichia coli (strain K12) EFP Protein (2-188 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length of Mature Protein.
Description : Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Beta-lysylation at Lys-34 is required for alleviation. The Pro codons and their context do not affect activity; only consecutive Pro residues (not another amino acid) are affected by EF-P. Has stimulatory effects on peptide bond formation between ribosome-bound initiator tRNA(fMet) and puromycin, and N-acetyl-Phe tRNA(Phe)-primed poly(U)-directed poly(Phe) synthesis.
Source : E. coli
Species : E. coli
Tag : His-SUMO
Form : Tris-based buffer,50% glycerol
Molecular Mass : 36.5 kDa
Protein length : 2-188 aa
AA Sequence : ATYYSNDFRA GLKIMLDGEP YAVEASEFVK PGKGQAFARV KLRRLLTGTR VEKTFKSTDS AEGADVVDMN LTYLYNDGEF WHFMNNETFE QLSADAKAIG DNAKWLLDQA ECIVTLWNGQ PISVTPPNFV ELEIVDTDPG LKGDTAGTGG KPATLSTGAV VKVPLFVQIG EVIKVDTRSG EYVSRVK
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : efp protein chain elongation factor EF-P [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol : EFP
Synonyms : ECK4141;
Gene ID : 948661
Protein Refseq : NP_418571
UniProt ID : P0A6N4

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