Recombinant Escherichia coli EFP Protein (2-188 aa), His-tagged

• Cat.No.: EFP-2123E
• Product Overview: Recombinant Escherichia coli (strain K12) EFP Protein (2-188 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Microbiology. Protein Description: Full Length of Mature Protein.

Specification

• Description: Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Beta-lysylation at Lys-34 is required for alleviation. The Pro codons and their context do not affect activity; only consecutive Pro residues (not another amino acid) are affected by EF-P. Has stimulatory effects on peptide bond formation between ribosome-bound initiator tRNA(fMet) and puromycin, and N-acetyl-Phe tRNA(Phe)-primed poly(U)-directed poly(Phe) synthesis.
• Source: Yeast
• Species: E. coli
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 22.5 kDa
• Protein length: 2-188 aa
• AA Sequence: ATYYSNDFRAGLKIMLDGEPYAVEA SEFVKPGKGQAFARVKLRRLLTGTR VEKTFKSTDSAEGADVVDMNLTYLY NDGEFWHFMNNETFEQLSADAKAIG DNAKWLLDQAECIVTLWNGQPISVT PPNFVELEIVDTDPGLKGDTAGTGG KPATLSTGAVVKVPLFVQIGEVIKV DTRSGEYVSRVK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: efp protein chain elongation factor EF-P [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: EFP
• Synonyms: efp; ECK4141
• Gene ID: 948661
• Protein Refseq: NP_418571
• UniProt ID: P0A6N4