Recombinant Full Length Human ABHD2 Protein, C-Flag-tagged

• Cat.No.: ABHD2-867HFL
• Product Overview: Recombinant Full Length Human ABHD2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein containing an alpha/beta hydrolase fold, which is a catalytic domain found in a wide range of enzymes. The encoded protein is an acylglycerol lipase that catalyzes the hydrolysis of endocannabinoid arachidonoylglycerol from the cell membrane. This leads to activation of the sperm calcium channel CatSper, which results in sperm activation. Alternative splicing of this gene results in two transcript variants encoding the same protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 48.1 kDa
• AA Sequence: MNAMLETPELPAVFDGVKLAAVAAV LYVIVRCLNLKSPTAPPDLYFQDSG LSRFLLKSCPLLTKEYIPPL IWGKSGHIQTALYGKMGRVRSPHPY GHRKFITMSDGATSTFDLFEPLAEH CVGDDITMVICPGIANHSEK QYIRTFVDYAQKNGYRCAVLNHLGA LPNIELTSPRMFTYGCTWEFGAMVN YIKKTYPLTQLVVVGFSLGG NIVCKYLGETQANQEKVLCCVSVCQ GYSALRAQETFMQWDQCQRFYNFLM ADNMKKIILSHRQALFGDHV KKPQSLEDTDLSRLYTATSLMQIDD NVMRKFHGYNSLKEYYEEESCMRYL HRIYVPLMLVNAADDPLVHE SLLTIPKSLSEKRENVMFVLPLHGG HLGFFEGSVLFPEPLTWMDKLVVEY ANAICQWERNKLQCSDTEQV
  EADLETRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: ABHD2 abhydrolase domain containing 2, acylglycerol lipase [ Homo sapiens (human) ]
• Official Symbol: ABHD2
• Synonyms: HS1-2; LABH2; PHPS1-2
• Gene ID: 11057
• mRNA Refseq: NM_007011.8
• Protein Refseq: NP_008942.3
• MIM: 612196
• UniProt ID: P08910