Recombinant Full Length Human AGO1 Protein, GST-tagged

• Cat.No.: AGO1-4569HF
• Product Overview: Human EIF2C1 full-length ORF ( NP_036331.1, 1 a.a. - 857 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 123.6 kDa
• Protein Length: 857 amino acids
• AA Sequence: MEAGPSGAAAGAYLPPLQQVFQAPR RPGIGTVGKPIKLLANYFEVDIPKI DVYHYEVDIKPDKCPRRVNREVVEY MVQHFKPQIFGDRKPVYDGKKNIYT VTALPIGNERVDFEVTIPGEGKDRI FKVSIKWLAIVSWRMLHEALVSGQI PVPLESVQALDVAMRHLASMRYTPV GRSFFSPPEGYYHPLGGGREVWFGF HQSVRPAMWKMMLNIDVSATAFYKA QPVIEFMCEVLDIRNIDEQPKPLTD SQRVRFTKEIKGLKVEVTHCGQMKR KYRVCNVTRRPASHQTFPLQLESGQ TVECTVAQYFKQKYNLQLKYPHLPC LQVGQEQKHTYLPLEVCNIVAGQRC IKKLTDNQTSTMIKATARSAPDRQE EISRLMKNASYNLDPYIQEFGIKVK DDMTEVTGRVLPAPILQYGGRNRAI ATPNQGVWDMRGKQFYNGIEIKVWA IACFAPQKQCREEVLKNFTDQLRKI SKDAGMPIQGQPCFCKYAQGADSVE PMFRHLKNTYSGLQLIIVILPGKTP VYAEVKRVGDTLLGMATQCVQVKNV VKTSPQTLSNLCLKINVKLGGINNI LVPHQRSAVFQQPVIFLGADVTHPP AGDGKKPSITAVVGSMDAHPSRYCA TVRVQRPRQEIIEDLSYMVRELLIQ FYKSTRFKPTRIIFYRDGVPEGQLP QILHYELLAIRDACIKLEKDYQPGI TYIVVQKRHHTRLFCADKNERIGKS GNIPAGTTVDTNITHPFEFDFYLCS HAGIQGTSRPSHYYVLWDDNRFTAD ELQILTYQLCHTYVRCTRSVSIPAP AYYARLVAFRARYHLVDKEHDSGEG SHISGQSNGRDPQALAKAVQVHQDT LRTMYFA
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: AGO1 argonaute 1, RISC catalytic component [ Homo sapiens (human) ]
• Official Symbol: AGO1
• Synonyms: AGO1; argonaute 1, RISC catalytic component; EIF2C1; eukaryotic translation initiation factor 2C, 1; protein argonaute-1; AGO1; argonaute 1; hAgo1; eIF2C 1; eIF-2C 1; argonaute1; putative RNA-binding protein Q99; Golgi Endoplasmic Reticulum protein 95 kDa; Q99; EIF2C; GERP95; DKFZp686M13167
• Gene ID: 26523
• mRNA Refseq: NM_012199
• Protein Refseq: NP_036331
• MIM: 606228
• UniProt ID: Q9UL18