Recombinant Full Length Human ALDH1A1 Protein, C-Flag-tagged

• Cat.No.: ALDH1A1-1999HFL
• Product Overview: Recombinant Full Length Human ALDH1A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.7 kDa
• AA Sequence: MSSSGTPDLPVLLTDLKIQYTKIFI NNEWHDSVSGKKFPVFNPATEEELC QVEEGDKEDVDKAVKAARQA FQIGSPWRTMDASERGRLLYKLADL IERDRLLLATMESMNGGKLYSNAYL NDLAGCIKTLRYCAGWADKI QGRTIPIDGNFFTYTRHEPIGVCGQ IIPWNFPLVMLIWKIGPALSCGNTV VVKPAEQTPLTALHVASLIK EAGFPPGVVNIVPGYGPTAGAAISS HMDIDKVAFTGSTEVGKLIKEAAGK SNLKRVTLELGGKSPCIVLA DADLDNAVEFAHHGVFYHQGQCCIA ASRIFVEESIYDEFVRRSVERAKKY ILGNPLTPGVTQGPQIDKEQ YDKILDLIESGKKEGAKLECGGGPW GNKGYFVQPTVFSNVTDEMRIAKEE IFGPVQQIMKFKSLDDVIKR ANNTFYGLSAGVFTKDIDKAITISS ALQAGTVWVNCYGVVSAQCPFGGFK MSGNGRELGEYGFHEYTEVK TVTVKISQKNS TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Metabolic pathways, Retinol metabolism

Gene Information

• Gene Name: ALDH1A1 aldehyde dehydrogenase 1 family member A1 [ Homo sapiens (human) ]
• Official Symbol: ALDH1A1
• Synonyms: ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
• Gene ID: 216
• mRNA Refseq: NM_000689.5
• Protein Refseq: NP_000680.2
• MIM: 100640
• UniProt ID: P00352