Recombinant Full Length Human ANAPC10 Protein, C-Flag-tagged

• Cat.No.: ANAPC10-2092HFL
• Product Overview: Recombinant Full Length Human ANAPC10 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 21.1 kDa
• AA Sequence: MTTPNKTPPGADPKQLERTGTVREI GSQAVWSLSSCKPGFGVDQLRDDNL ETYWQSDGSQPHLVNIQFRR KTTVKTLCIYADYKSDESYTPSKIS VRVGNNFHNLQEIRQLELVEPSGWI HVPLTDNHKKPTRTFMIQIA VLANHQNGRDTHMRQIKIYTPVEES SIGKFPRCTTIDFMMYRSIR TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: ANAPC10 anaphase promoting complex subunit 10 [ Homo sapiens (human) ]
• Official Symbol: ANAPC10
• Synonyms: DOC1; APC10
• Gene ID: 10393
• mRNA Refseq: NM_014885.5
• Protein Refseq: NP_055700.2
• MIM: 613745
• UniProt ID: Q9UM13