Recombinant Full Length Human ARHGDIB Protein, C-Flag-tagged

• Cat.No.: ARHGDIB-63HFL
• Product Overview: Recombinant Full Length Human ARHGDIB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 22.8 kDa
• AA Sequence: MTEKAPEPHVEEDDDDELDSKLNYK PPPQKSLKELQEMDKDDESLIKYKK TLLGDGPVVTDPKAPNVVVT RLTLVCESAPGPITMDLTGDLEALK KETIVLKEGSEYRVKIHFKVNRDIV SGLKYVQHTYRTGVKVDKAT
  FMVGSYGPRPEEYEFLTPVEEAPKG MLARGTYHNKSFFTDDDKQDHLSWE WNLSIKKEWTETRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway

Gene Information

• Gene Name: ARHGDIB Rho GDP dissociation inhibitor beta [ Homo sapiens (human) ]
• Official Symbol: ARHGDIB
• Synonyms: D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2
• Gene ID: 397
• mRNA Refseq: NM_001175.7
• Protein Refseq: NP_001166.3
• MIM: 602843
• UniProt ID: P52566