Recombinant Full Length Human ATIC Protein, C-Flag-tagged

• Cat.No.: ATIC-1689HFL
• Product Overview: Recombinant Full Length Human ATIC Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 64.4 kDa
• AA Sequence: MAPGQLALFSVSDKTGLVEFARNLT ALGLNLVASGGTAKALRDAGLAVRD VSELTGFPEMLGGRVKTLHP AVHAGILARNIPEDNADMARLDFNL IRVVACNLYPFVKTVASPGVTVEEA VEQIDIGGVTLLRAAAKNHA RVTVVCEPEDYVVVSTEMQSSESKD TSLETRRQLALKAFTHTAQYDEAIS DYFRKQYSKGVSQMPLRYGM NPHQTPAQLYTLQPKLPITVLNGAP GFINLCDALNAWQLVKELKEALGIP AAASFKHVSPAGAAVGIPLS EDEAKVCMVYDLYKTLTPISAAYAR ARGADRMSSFGDFVALSDVCDVPTA KIISREVSDGIIAPGYEEEA LTILSKKKNGNYCVLQMDQSYKPDE NEVRTLFGLHLSQKRNNGVVDKSLF SNVVTKNKDLPESALRDLIV ATIAVKYTQSNSVCYAKNGQVIGIG AGQQSRIHCTRLAGDKANYWWLRHH PQVLSMKFKTGVKRAEISNA IDQYVTGTIGEDEDLIKWKALFEEV PELLTEAEKKEWVEKLTEVSISSDA FFPFRDNVDRAKRSGVAYIA
  APSGSAADKVVIEACDELGIILAHT NLRLFHHTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism

Gene Information

• Gene Name: ATIC 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase [ Homo sapiens (human) ]
• Official Symbol: ATIC
• Synonyms: PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p
• Gene ID: 471
• mRNA Refseq: NM_004044.7
• Protein Refseq: NP_004035.2
• MIM: 601731
• UniProt ID: P31939