Recombinant Human ATIC protein, GST-tagged
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|Product Overview :||Recombinant Human ATIC protein(NP_004035.2)(291-592 aa) was fused to GST-tagged and expressed in E. coli.|
|Description :||This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.|
|Source :||E. coli|
|Form :||The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4), 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.|
|Bio-activity :||Not tested.|
|Protein length :||291-592 aa|
|AA Sequence :||DLYKTLTPISAAYAR ARGADRMSSFGDFVA LSDVCDVPTAKIISR EVSDGIIAPGYEEEA LTILSKKKNGNYCVL QMDQSYKPDENEVRT LFGLHLSQKRNNGVV DKSLFSNVVTKNKDL PESALRDLIVATIAV KYTQSNSVCYAKNGQ VIGIGAGQQSRIHCT RLAGDKANYWWLRHH PQVLSMKFKTGVKRA EISNAIDQYVTGTIG EDEDLIKWKALFEEV PELLTEAEKKEWVEK LTEVSISSDAFFPFR DNVDRAKRSGVAYIA APSGSAADKVVIEAC DELGIILAHTNLRLF HH|
|Endotoxin :||<1.0 EU per μg protein as determined by the LAL method.|
|Purity :||90%, by SDS-PAGE with Coomassie Brilliant Blue staining.|
|Applications :||Blocking peptide|
|Stability :||Store for up to 12 months at -20°C to -80°C as lyophilized powder.|
|Storage :||Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.|
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature(see below).|
|SDS-PAGE of ATIC-25H :||
|Gene Name :||ATIC|
|Official Symbol :||ATIC|
|Synonyms :||AICAR; AICARFT; FLJ93545; IMPCHASE; PURH|
|Gene ID :||471|
|mRNA Refseq :||NM_004044.7|
|Protein Refseq :||NP_004035.2|
|UniProt ID :||P31939|
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