Recombinant Human ATIC protein, GST-tagged ATIC-25H

Recombinant Human ATIC protein, GST-tagged

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Recombinant Human ATIC protein, GST-tagged

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Cat.No. : ATIC-25H
Product Overview : Recombinant Human ATIC protein(NP_004035.2)(291-592 aa) was fused to GST-tagged and expressed in E. coli.
Description : This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.
Source : E. coli
Species : Human
Tag : GST
Form : The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4), 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
Bio-activity : Not tested.
Protein length : 291-592 aa
AA Sequence : DLYKTLTPISAAYAR ARGADRMSSFGDFVA LSDVCDVPTAKIISR EVSDGIIAPGYEEEA LTILSKKKNGNYCVL QMDQSYKPDENEVRT LFGLHLSQKRNNGVV DKSLFSNVVTKNKDL PESALRDLIVATIAV KYTQSNSVCYAKNGQ VIGIGAGQQSRIHCT RLAGDKANYWWLRHH PQVLSMKFKTGVKRA EISNAIDQYVTGTIG EDEDLIKWKALFEEV PELLTEAEKKEWVEK LTEVSISSDAFFPFR DNVDRAKRSGVAYIA APSGSAADKVVIEAC DELGIILAHTNLRLF HH
Endotoxin : <1.0 EU per μg protein as determined by the LAL method.
Purity : 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Applications : Blocking peptide
Stability : Store for up to 12 months at -20°C to -80°C as lyophilized powder.
Storage : Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Reconstitution : Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature(see below).
SDS-PAGE of ATIC-25H :

Gene Name : ATIC
Official Symbol : ATIC
Synonyms : AICAR; AICARFT; FLJ93545; IMPCHASE; PURH
Gene ID : 471
mRNA Refseq : NM_004044.7
Protein Refseq : NP_004035.2
MIM : 601731
UniProt ID : P31939

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