Recombinant Full Length Human ATP5F1B Protein, C-Flag-tagged

• Cat.No.: ATP5F1B-758HFL
• Product Overview: Recombinant Full Length Human ATP5F1B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.7 kDa
• AA Sequence: MLGFVGRVAAAPASGALRRLTPSAS LPPAQLLLRAAPTAVHPVRDYAAQT SPSPKAGAATGRIVAVIGAV VDVQFDEGLPPILNALEVQGRETRL VLEVAQHLGESTVRTIAMDGTEGLV RGQKVLDSGAPIKIPVGPET LGRIMNVIGEPIDERGPIKTKQFAP IHAEAPEFMEMSVEQEILVTGIKVV DLLAPYAKGGKIGLFGGAGV GKTVLIMELINNVAKAHGGYSVFAG VGERTREGNDLYHEMIESGVINLKD ATSKVALVYGQMNEPPGARA RVALTGLTVAEYFRDQEGQDVLLFI DNIFRFTQAGSEVSALLGRIPSAVG YQPTLATDMGTMQERITTTK KGSITSVQAIYVPADDLTDPAPATT FAHLDATTVLSRAIAELGIYPAVDP LDSTSRIMDPNIVGSEHYDV ARGVQKILQDYKSLQDIIAILGMDE LSEEDKLTVSRARKIQRFLSQPFQV AEVFTGHMGKLVPLKETIKG
  FQQILAGEYDHLPEQAFYMVGPIEE AVAKADKLAEEHSSTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease

Gene Information

• Gene Name: ATP5F1B ATP synthase F1 subunit beta [ Homo sapiens (human) ]
• Official Symbol: ATP5F1B
• Synonyms: ATP5B; ATPMB; ATPSB; HUMOP2; HEL-S-271
• Gene ID: 506
• mRNA Refseq: NM_001686.4
• Protein Refseq: NP_001677.2
• MIM: 102910
• UniProt ID: P06576