Human ATP5F1B Knockdown Cell Lysate

• Cat.No.: ATP5F1B-063HKCL
• Product Overview: WB-validated ATP5F1B Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 57 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg ATP5F1B KD HeLa cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: ATP5F1B ATP synthase F1 subunit beta [ Homo sapiens (human) ]
• Official Symbol: ATP5F1B
• Synonyms: ATP5F1B; ATP synthase F1 subunit beta; ATP5B; ATPMB; ATPSB; HUMOP2; HEL-S-271; ATP synthase F(1) complex subunit beta, mitochondrial; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; epididymis secretory protein Li 271; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit; EC 7.1.2.2
• Gene ID: 506
• mRNA Refseq: NM_001686
• Protein Refseq: NP_001677
• MIM: 102910
• UniProt ID: P06576