Recombinant Human ATP5F1B Protein (48-529 aa), His-tagged

• Cat.No.: ATP5F1B-2167H
• Product Overview: Recombinant Human ATP5F1B Protein (48-529 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Tags & Cell Markers. Protein Description: Full Length of Mature Protein.

Specification

• Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 53.8 kDa
• Protein length: 48-529 aa
• AA Sequence: AQTSPSPKAGAATGRIVAVIGAVVD VQFDEGLPPILNALEVQGRETRLVL EVAQHLGESTVRTIAMDGTEGLVRG QKVLDSGAPIKIPVGPETLGRIMNV IGEPIDERGPIKTKQFAPIHAEAPE FMEMSVEQEILVTGIKVVDLLAPYA KGGKIGLFGGAGVGKTVLIMELINN VAKAHGGYSVFAGVGERTREGNDLY HEMIESGVINLKDATSKVALVYGQM NEPPGARARVALTGLTVAEYFRDQE GQDVLLFIDNIFRFTQAGSEVSALL GRIPSAVGYQPTLATDMGTMQERIT TTKKGSITSVQAIYVPADDLTDPAP ATTFAHLDATTVLSRAIAELGIYPA VDPLDSTSRIMDPNIVGSEHYDVAR GVQKILQDYKSLQDIIAILGMDELS EEDKLTVSRARKIQRFLSQPFQVAE VFTGHMGKLVPLKETIKGFQQILAG EYDHLPEQAFYMVGPIEEAVAKADK LAEEHSS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: ATP5F1B ATP synthase F1 subunit beta [ Homo sapiens (human) ]
• Official Symbol: ATP5F1B
• Synonyms: ATP5B; ATPMB; ATPSB; HEL-S-271
• Gene ID: 506
• mRNA Refseq: NM_001686
• Protein Refseq: NP_001677
• UniProt ID: P06576