Recombinant Full Length Human ATXN3 Protein, C-Flag-tagged

• Cat.No.: ATXN3-1896HFL
• Product Overview: Recombinant Full Length Human ATXN3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 12-44 to 52-86 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 41.1 kDa
• AA Sequence: MESIFHEKQEGSLCAQHCLNNLLQG EYFSPVELSSIAHQLDEEERMRMAE GGVTSEDYRTFLQQPSGNMD DSGFFSIQVISNALKVWGLELILFN SPEYQRLRIDPINERSFICNYKEHW FTVRKLGKQWFNLNSLLTGP ELISDTYLALFLAQLQQEGYSIFVV KGDLPDCEADQLLQMIRVQQMHRPK LIGEELAQLKEQRVHKTDLE RVLEANDGSGMLDEDEEDLQRALAL SRQEIDMEDEEADLRRAIQLSMQGS SRNISQDMTQTSGTNLTSEE LRKRREAYFEKQQQKQQQQQQQQQQ GDLSGQSSHPCERPATSSGALGSDL GDAMSEEDMLQAAVTMSLET VRNDLKTEGKK TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: ATXN3 ataxin 3 [ Homo sapiens (human) ]
• Official Symbol: ATXN3
• Synonyms: AT3; JOS; MJD; ATX3; MJD1; SCA3
• Gene ID: 4287
• mRNA Refseq: NM_004993.6
• Protein Refseq: NP_004984.2
• MIM: 607047
• UniProt ID: P54252