| Description : |
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq |
| Source : |
In Vitro Cell Free System |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Molecular Mass : |
88.99 kDa |
| Protein Length : |
575 amino acids |
| AA Sequence : |
MSAATHSPMMQVASGNGDRDPLPPG WEIKIDPQTGWPFFVDHNSRTTTWN DPRVPSEGPKETPSSANGPSREGSR LPPAREGHPVYPQLRPGYIPIPVLH EGAENRQVHPFHVYPQPGMQRFRTE AAAAAPQRSQSPLRGMPETTQPDKQ CGQVAAAAAAQPPASHGPERSQSPA ASDCSSSSSSASLPSSGRSSLGSHQ LPRGYISIPVIHEQNVTRPAAQPSF HQAQKTHYPAQQGEYQTHQPVYHKI QGDDWEPRPLRAASPFRSSVQGASS REGSPARSSTPLHSPSPIRVHTVVD RPQQPMTHRETAPVSQPENKPESKP GPVGPELPPGHIPIQVIRKEVDSKP VSQKPPPPSEKVEVKVPPAPVPCPP PSPGPSAVPSSPKSVATEERAAPST APAEATPPKPGEAEAPPKHPGVLKV EAILEKVQGLEQAVDNFEGKKTDKK YLMIEEYLTKELLALDSVDPEGRAD VRQARRDGVRKVQTILEKLEQKAID VPGQVQVYELQPSNLEADQPLQAIM EMGAVAADKGKKNAGNAEDPHTETQ QPEATAAATSNPSSMTDTPGNPAAP |
| Notes : |
Best use within three months from the date of receipt of this protein. |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
