Human BAG3 Knockdown Cell Lysate

• Cat.No.: BAG3-326HKCL
• Product Overview: WB-validated BAG3 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 62 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg BAG3 KD HeLa cell lysate
• Protein Families: Druggable Genome
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: BAG3 BCL2-associated athanogene 3 [ Homo sapiens (human) ]
• Official Symbol: BAG3
• Synonyms: BAG3; BCL2-associated athanogene 3; BAG family molecular chaperone regulator 3; docking protein CAIR-1; BCL2-binding athanogene 3; bcl-2-binding protein Bis; bcl-2-associated athanogene 3; BAG-family molecular chaperone regulator-3; BIS; BAG-3; CAIR-1; MGC104307
• Gene ID: 9531
• mRNA Refseq: NM_004281
• Protein Refseq: NP_004272
• MIM: 603883
• UniProt ID: O95817