Recombinant Full Length Human CBL Protein, C-Flag-tagged

• Cat.No.: CBL-808HFL
• Product Overview: Recombinant Full Length Human CBL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 99.5 kDa
• AA Sequence: MAGNVKKSSGAGGGSGSGGSGSGGL IGLMKDAFQPHHHHHHHLSPHPPGT VDKKMVEKCWKLMDKVVRLC QNPKLALKNSPPYILDLLPDTYQHL RTILSRYEGKMETLGENEYFRVFME NLMKKTKQTISLFKEGKERM YEENSQPRRNLTKLSLIFSHMLAEL KGIFPSGLFQGDTFRITKADAAEFW RKAFGEKTIVPWKSFRQALH EVHPISSGLEAMALKSTIDLTCNDY ISVFEFDIFTRLFQPWSSLLRNWNS LAVTHPGYMAFLTYDEVKAR LQKFIHKPGSYIFRLSCTRLGQWAI GYVTADGNILQTIPHNKPLFQALID GFREGFYLFPDGRNQNPDLT GLCEPTPQDHIKVTQEQYELYCEMG STFQLCKICAENDKDVKIEPCGHLM CTSCLTSWQESEGQGCPFCR CEIKGTEPIVVDPFDPRGSGSLLRQ GAEGAPSPNYDDDDDERADDTLFMM KELAGAKVERPPSPFSMAPQ ASLPPVPPRLDLLPQRVCVPSSASA LGTASKAASGSLHKDKPLPVPPTLR DLPPPPPPDRPYSVGAESRP QRRPLPCTPGDCPSRDKLPPVPSSR LGDSWLPRPIPKVPVSAPSSSDPWT GRELTNRHSLPFSLPSQMEP RPDVPRLGSTFSLDTSMSMNSSPLV GPECDHPKIKPSSSANAIYSLAARP LPVPKLPPGEQCEGEEDTEY MTPSSRPLRPLDTSQSSRACDCDQQ IDSCTYEAMYNIQSQAPSITESSTF GEGNLAAAHANTGPEESENE DDGYDVPKPPVPAVLARRTLSDISN ASSSFGWLSLDGDPTTNVTEGSQVP ERPPKPFPRRINSERKAGSC QQGSGPAASAATASPQLSSEIENLM SQGYSYQDIQKALVIAQNNIEMAKN ILREFVSISSPAHVATTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: CBL Cbl proto-oncogene [ Homo sapiens (human) ]
• Official Symbol: CBL
• Synonyms: CBL2; NSLL; C-CBL; RNF55; FRA11B
• Gene ID: 867
• mRNA Refseq: NM_005188.4
• Protein Refseq: NP_005179.2
• MIM: 165360
• UniProt ID: P22681