Recombinant Full Length Human CRYAB Protein, C-Flag-tagged

Cat.No. : CRYAB-1060HFL
Product Overview : Recombinant Full Length Human CRYAB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.
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Description : Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.
Source : Mammalian cells
Species : Human
Tag : Flag
Form : 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Mass : 20 kDa
AA Sequence : MDIAIHHPWIRRPFFPFHSPSRLFD QFFGEHLLESDLFPTSTSLSPFYLR PPSFLRAPSWFDTGLSEMRL EKDRFSVNLDVKHFSPEELKVKVLG DVIEVHGKHEERQDEHGFISREFHR KYRIPADVDPLTITSSLSSD
GVLTVNGPRKQVSGPERTIPITREE KPAVTAAPKKTRTRPLEQKLISEED LAANDILDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining.
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade.
Concentration : >50 ug/mL as determined by microplate BCA method.
Preparation : Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
SDS-PAGE: Coomassie blue staining of purified CRYAB protein.
Gene Name : CRYAB crystallin alpha B [ Homo sapiens (human) ]
Official Symbol : CRYAB
Synonyms : MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
Gene ID : 1410
mRNA Refseq : NM_001885.3
Protein Refseq : NP_001876.1
MIM : 123590
UniProt ID : P02511

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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