Recombinant Full Length Human CUL4A Protein, C-Flag-tagged

• Cat.No.: CUL4A-1687HFL
• Product Overview: Recombinant Full Length Human CUL4A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 76.6 kDa
• AA Sequence: MADEAPRKGSFSALVGRTNGLTKPA ALAAAPAKPGGAGGSKKLVIKNFRD RPRLPDNYTQDTWRKLHEAV RAVQSSTSIRYNLEELYQAVENLCS HKVSPMLYKQLRQACEDHVQAQILP FREDSLDSVLFLKKINTCWQ DHCRQMIMIRSIFLFLDRTYVLQNS TLPSIWDMGLELFRTHIISDKMVQS KTIDGILLLIERERSGEAVD RSLLRSLLGMLSDLQVYKDSFELKF LEETNCLYAAEGQRLMQEREVPEYL NHVSKRLEEEGDRVITYLDH STQKPLIACVEKQLLGEHLTAILQK GLDHLLDENRVPDLAQMYQLFSRVR GGQQALLQHWSEYIKTFGTA IVINPEKDKDMVQDLLDFKDKVDHV IEVCFQKNERFVNLMKESFETFINK RPNKPAELIAKHVDSKLRAG NKEATDEELERTLDKIMILFRFIHG KDVFEAFYKKDLAKRLLVGKSASVD AEKSMLSKLKHECGAAFTSK LEGMFKDMELSKDIMVHFKQHMQNQ SDSGPIDLTVNILTMGYWPTYTPME VHLTPEMIKLQEVFKAFYLG KHSGRKLQWQTTLGHAVLKAEFKEG KKEFQVSLFQTLVLLMFNEGDGFSF EEIKMATGIEDSELRRTLQS LACGKARVLIKSPKGKEVEDGDKFI FNGEFKHKLFRIKINQIQMKETVEE QVSTTERVFQDRQYQIDAAI
  VRIMKMRKTLGHNLLVSELYNQLKF PVKPGDLKKRIESLIDRDYMERDKD NPNQYHYVATRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: CUL4A cullin 4A [ Homo sapiens (human) ]
• Official Symbol: CUL4A
• Synonyms: cullin 4A; OTTHUMP00000018753; OTTHUMP00000018754
• Gene ID: 8451
• mRNA Refseq: NM_003589.4
• Protein Refseq: NP_003580.1
• MIM: 603137
• UniProt ID: Q13619