Recombinant Full Length Human CYTH1 Protein, C-Flag-tagged

• Cat.No.: CYTH1-1881HFL
• Product Overview: Recombinant Full Length Human CYTH1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.2 kDa
• AA Sequence: MEEDDSYVPSDLTAEERQELENIRR RKQELLADIQRLKDEIAEVANEIEN LGSTEERKNMQRNKQVAMGR KKFNMDPKKGIQFLIENDLLKNTCE DIAQFLYKGEGLNKTAIGDYLGERD EFNIQVLHAFVELHEFTDLN LVQALRQFLWSFRLPGEAQKIDRMM EAFAQRYCQCNNGVFQSTDTCYVLS FAIIMLNTSLHNPNVKDKPT VERFIAMNRGINDGGDLPEELLRNL YESIKNEPFKIPEDDGNDLTHTFFN PDREGWLLKLGGGRVKTWKR RWFILTDNCLYYFEYTTDKEPRGII PLENLSIREVEDSKKPNCFELYIPD NKDQVIKACKTEADGRVVEG NHTVYRISAPTPEEKEEWIKCIKAA ISRDPFYEMLAARKKKVSSTKRH TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: CYTH1 cytohesin 1 [ Homo sapiens (human) ]
• Official Symbol: CYTH1
• Synonyms: B2-1; SEC7; PSCD1; D17S811E; CYTOHESIN-1
• Gene ID: 9267
• mRNA Refseq: NM_004762.6
• Protein Refseq: NP_004753.1
• MIM: 182115
• UniProt ID: Q15438