Recombinant Full Length Human DGKA Protein, C-Flag-tagged

• Cat.No.: DGKA-627HFL
• Product Overview: Recombinant Full Length Human DGKA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 82.5 kDa
• AA Sequence: MAKERGLISPSDFAQLQKYMEYSTK KVSDVLKLFEDGEMAKYVQGDAIGY EGFQQFLKIYLEVDNVPRHL SLALFQSFETGHCLNETNVTKDVVC LNDVSCYFSLLEGGRPEDKLEFTFK LYDTDRNGILDSSEVDKIIL QMMRVAEYLDWDVSELRPILQEMMK EIDYDGSGSVSQAEWVRAGATTVPL LVLLGLEMTLKDDGQHMWRP KRFPRPVYCNLCESSIGLGKQGLSC NLCKYTVHDQCAMKALPCEVSTYAK SRKDIGVQSHVWVRGGCESG RCDRCQKKIRIYHSLTGLHCVWCHL EIHDDCLQAVGHECDCGLLRDHILP PSSIYPSVLASGPDRKNSKT SQKTMDDLNLSTSEALRIDPVPNTH PLLVFVNPKSGGKQGQRVLWKFQYI LNPRQVFNLLKDGPEIGLRL FKDVPDSRILVCGGDGTVGWILETI DKANLPVLPPVAVLPLGTGNDLARC LRWGGGYEGQNLAKILKDLE MSKVVHMDRWSVEVIPQQTEEKSDP VPFQIINNYFSIGVDASIAHRFHIM REKYPEKFNSRMKNKLWYFE FATSESIFSTCKKLEESLTVEICGK PLDLSNLSLEGIAVLNIPSMHGGSN LWGDTRRPHGDIYGINQALG ATAKVITDPDILKTCVPDLSDKRLE VVGLEGAIEMGQIYTKLKNAGRRLA KCSEITFHTTKTLPMQIDGE
  PWMQTPCTIKITHKNQMPMLMGPPP RSTNFFGFLSTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system

Gene Information

• Gene Name: DGKA diacylglycerol kinase alpha [ Homo sapiens (human) ]
• Official Symbol: DGKA
• Synonyms: DAGK; DAGK1; DGK-alpha
• Gene ID: 1606
• mRNA Refseq: NM_201444.3
• Protein Refseq: NP_958852.1
• MIM: 125855
• UniProt ID: P23743