Recombinant Full Length Human DHX36 Protein, C-Flag-tagged

• Cat.No.: DHX36-425HFL
• Product Overview: Recombinant Full Length Human DHX36 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 114.6 kDa
• AA Sequence: MSYDYHQNWGRDGGPRSSGGGYGGG PAGGHGGNRGSGGGGGGGGGGRGGR GRHPGHLKGREIGMWYAKKQ GQKNKEAERQERAVVHMDERREEQI VQLLNSVQAKNDKESEAQISWFAPE DHGYGTEVSTKNTPCSENKL DIQEKKLINQEKKMFRIRNRSYIDR DSEYLLQENEPDGTLDQKLLEDLQK KKNDLRYIEMQHFREKLPSY GMQKELVNLIDNHQVTVISGETGCG KTTQVTQFILDNYIERGKGSACRIV CTQPRRISAISVAERVAAER AESCGSGNSTGYQIRLQSRLPRKQG SILYCTTGIILQWLQSDPYLSSVSH IVLDEIHERNLQSDVLMTVV KDLLNFRSDLKVILMSATLNAEKFS EYFGNCPMIHIPGFTFPVVEYLLED VIEKIRYVPEQKEHRCQFKR GFMQGHVNRQEKEEKEAIYKERWPD YVRELRRRYSASTVDVIEMMEDDKV DLNLIVALIRYIVLEEEDGA ILVFLPGWDNISTLHDLLMSQVMFK SDKFLIIPLHSLMPTVNQTQVFKRT PPGVRKIVIATNIAETSITI DDVVYVIDGGKIKETHFDTQNNIST MSAEWVSKANAKQRKGRAGRVQPGH CYHLYNGLRASLLDDYQLPE ILRTPLEELCLQIKILRLGGIAYFL SRLMDPPSNEAVLLSIRHLMELNAL DKQEELTPLGVHLARLPVEP HIGKMILFGALFCCLDPVLTIAASL SFKDPFVIPLGKEKIADARRKELAK DTRSDHLTVVNAFEGWEEAR RRGFRYEKDYCWEYFLSSNTLQMLH NMKGQFAEHLLGAGFVSSRNPKDPE SNINSDNEKIIKAVICAGLY PKVAKIRLNLGKKRKMVKVYTKTDG LVAVHPKSVNVEQTDFHYNWLIYHL KMRTSSIYLYDCTEVSPYCL LFFGGDISIQKDNDQETIAVDEWIV FQSPARIAHLVKELRKELDILLQEK IESPHPVDWNDTKSRDCAVL
  SAIIDLIKTQEKATPRNFPPRFQDG YYSTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DHX36 DEAH-box helicase 36 [ Homo sapiens (human) ]
• Official Symbol: DHX36
• Synonyms: G4R1; RHAU; DDX36; MLEL1
• Gene ID: 170506
• mRNA Refseq: NM_020865.3
• Protein Refseq: NP_065916.2
• MIM: 612767
• UniProt ID: Q9H2U1