Recombinant Full Length Human DIAPH1 Protein, C-Flag-tagged

• Cat.No.: DIAPH1-720HFL
• Product Overview: Recombinant Full Length Human DIAPH1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 140.1 kDa
• AA Sequence: MEPPGGSLGPGRGTRDKKKGRSPDE LPSAGGDGGKSKKFLERFTSMRIKK EKEKPNSAHRNSSASYGDDP TAQSLQDVSDEQVLVLFEQMLLDMN LNEEKQQPLREKDIIIKREMVSQYL YTSKAGMSQKESSKSAMMYI QELRSGLRDMPLLSCLESLRVSLNN NPVSWVQTFGAEGLASLLDILKRLH DEKEETAGSYDSRNKHEIIR CLKAFMNNKFGIKTMLETEEGILLL VRAMDPAVPNMMIDAAKLLSALCIL PQPEDMNERVLEAMTERAEM DEVERFQPLLDGLKSGTTIALKVGC LQLINALITPAEELDFRVHIRSELM RLGLHQVLQDLREIENEDMR VQLNVFDEQGEEDSYDLKGRLDDIR MEMDDFNEVFQILLNTVKDSKAEPH FLSILQHLLLVRNDYEARPQ YYKLIEECISQIVLHKNGADPDFKC RHLQIEIEGLIDQMIDKTKVEKSEA KAAELEKKLDSELTARHELQ VEMKKMESDFEQKLQDLQGEKDALH SEKQQIATEKQDLEAEVSQLTGEVA KLTKELEDAKKEMASLSAAA ITVPPSVPSRAPVPPAPPLPGDSGT IIPPPPAPGDSTTPPPPPPPPPPPP LPGGVCISSPPSLPGGTAIS PPPPLSGDATIPPPPPLPEGVGIPS PSSLPGGTAIPPPPPLPGSARIPPP PPPLPGSAGIPPPPPPLPGE AGMPPPPPPLPGGPGIPPPPPFPGG PGIPPPPPGMGMPPPPPFGFGVPAA PVLPFGLTPKKLYKPEVQLR RPNWSKLVAEDLSQDCFWTKVKEDR FENNELFAKLTLTFSAQTKTSKAKK DQEGGEEKKSVQKKKVKELK VLDSKTAQNLSIFLGSFRMPYQEIK NVILEVNEAVLTESMIQNLIKQMPE PEQLKMLSELKDEYDDLAES EQFGVVMGTVPRLRPRLNAILFKLQ FSEQVENIKPEIVSVTAACEELRKS ESFSNLLEITLLVGNYMNAG SRNAGAFGFNISFLCKLRDTKSTDQ KMTLLHFLAELCENDYPDVLKFPDE LAHVEKASRVSAENLQKNLD QMKKQISDVERDVQNFPAATDEKDK FVEKMTSFVKDAQEQYNKLRMMHSN METLYKELGEYFLFDPKKLS VEEFFMDLHNFRNMFLQAVKENQKR RETEEKMRRAKLAKEKAEKERLEKQ QKREQLIDMNAEGDETGVMD SLLEALQSGAAFRRKRGPRQANRKA GCAVTSLLASELTKDDAMAAVPAKV SKNSETFPTILEEAKELVGR
  ASTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton

Gene Information

• Gene Name: DIAPH1 diaphanous related formin 1 [ Homo sapiens (human) ]
• Official Symbol: DIAPH1
• Synonyms: DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1; mDia1
• Gene ID: 1729
• mRNA Refseq: NM_001079812.3
• Protein Refseq: NP_001073280.1
• MIM: 602121
• UniProt ID: O60610