Human DIAPH1 Knockdown Cell Lysate

• Cat.No.: DIAPH1-136HKCL
• Product Overview: WB-validated DIAPH1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 141 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg DIAPH1 KD HeLa cell lysate
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DIAPH1 diaphanous homolog 1 (Drosophila) [ Homo sapiens (human) ]
• Official Symbol: DIAPH1
• Synonyms: DIAPH1; diaphanous homolog 1 (Drosophila); DFNA1, diaphanous (Drosophila, homolog) 1; protein diaphanous homolog 1; hDIA1; LFHL1; diaphanous-related formin 1; diaphanous-related formin-1; DIA1; DRF1; DFNA1; FLJ25265
• Gene ID: 1729
• mRNA Refseq: NM_001079812
• Protein Refseq: NP_001073280
• MIM: 602121
• UniProt ID: O60610