Recombinant Full Length Human DNAJA2 Protein, C-Flag-tagged

• Cat.No.: DNAJA2-1657HFL
• Product Overview: Recombinant Full Length Human DNAJA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 45.6 kDa
• AA Sequence: MANVADTKLYDILGVPPGASENELK KAYRKLAKEYHPDKNPNAGDKFKEI SFAYEVLSNPEKRELYDRYG EQGLREGSGGGGGMDDIFSHIFGGG LFGFMGNQSRSRNGRRRGEDMMHPL KVSLEDLYNGKTTKLQLSKN VLCSACSGQGGKSGAVQKCSACRGR GVRIMIRQLAPGMVQQMQSVCSDCN GEGEVINEKDRCKKCEGKKV IKEVKILEVHVDKGMKHGQRITFTG EADQAPGVEPGDIVLLLQEKEHEVF QRDGNDLHMTYKIGLVEALC GFQFTFKHLDGRQIVVKYPPGKVIE PGCVRVVRGEGMPQYRNPFEKGDLY IKFDVQFPENNWINPDKLSE
  LEDLLPSRPEVPNIIGETEEVELQE FDSTRGSGGGQRREAYNDSSDEESS SHHGPGVQCAHQTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DNAJA2 DnaJ heat shock protein family (Hsp40) member A2 [ Homo sapiens (human) ]
• Official Symbol: DNAJA2
• Synonyms: DJ3; CPR3; DJA2; DNAJ; DNJ3; RDJ2; HIRIP4; PRO3015
• Gene ID: 10294
• mRNA Refseq: NM_005880.4
• Protein Refseq: NP_005871.1
• MIM: 611322
• UniProt ID: O60884