Recombinant Full Length Human DNAJA3 Protein, C-Flag-tagged

• Cat.No.: DNAJA3-1700HFL
• Product Overview: Recombinant Full Length Human DNAJA3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 52.3 kDa
• AA Sequence: MAARCSTRWLLVVVGTPRLPAISGR GARPPREGVVGAWLSRKLSVPAFAS SLTSCGPRALLTLRPGVSLT GTKHYPFICTASFHTSAPLAKEDYY QILGVPRNASQKEIKKAYYQLAKKY HPDTNKDDPKAKEKFSQLAE AYEVLSDEVKRKQYDAYGSAGFDPG ASGSQHSYWKGGPTVDPEELFRKIF GEFSSSSFGDFQTVFDQPQE YFMELTFNQAAKGVNKEFTVNIMDT CERCNGKGNEPGTKVQHCHYCGGSG METINTGPFVMRSTCRRCGG RGSIIISPCVVCRGAGQAKQKKRVM IPVPAGVEDGQTVRMPVGKREIFIT FRVQKSPVFRRDGADIHSDL FISIAQALLGGTARAQGLYETINVT IPPGTQTDQKIRMGGKGIPRINSYG YGDHYIHIKIRVPKRLTSRQ
  QSLILSYAEDETDVEGTVNGVTLTS SGGSTMDSSAGSKARREAGEDEEGF LSKLKKMFTSTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DNAJA3 DnaJ heat shock protein family (Hsp40) member A3 [ Homo sapiens (human) ]
• Official Symbol: DNAJA3
• Synonyms: TID1; HCA57; hTID-1
• Gene ID: 9093
• mRNA Refseq: NM_005147.6
• Protein Refseq: NP_005138.3
• MIM: 608382
• UniProt ID: Q96EY1