Recombinant Full Length Human EIF3D Protein, C-Flag-tagged

• Cat.No.: EIF3D-1694HFL
• Product Overview: Recombinant Full Length Human EIF3D Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 63.8 kDa
• AA Sequence: MAKFMTPVIQDNPSGWGPCAVPEQF RDMPYQPFSKGDRLGKVADWTGATY QDKRYTNKYSSQFGGGSQYA YFHEEDESSFQLVDTARTQKTAYQR NRMRFAQRNLRRDKDRRNMLQFNLQ ILPKSAKQKERERIRLQKKF QKQFGVRQKWDQKSQKPRDSSVEVR SDWEVKEEMDFPQLMKMRYLEVSEP QDIECCGALEYYDKAFDRIT TRSEKPLRSIKRIFHTVTTTDDPVI RKLAKTQGNVFATDAILATLMSCTR SVYSWDIVVQRVGSKLFFDK RDNSDFDLLTVSETANEPPQDEGNS FNSPRNLAMEATYINHNFSQQCLRM GKERYNFPNPNPFVEDDMDK NEIASVAYRYRRWKLGDDIDLIVRC EHDGVMTGANGEVSFINIKTLNEWD SRHCNGVDWRQKLDSQRGAV IATELKNNSYKLARWTCCALLAGSE YLKLGYVSRYHVKDSSRHVILGTQQ FKPNEFASQINLSVENAWGI
  LRCVIDICMKLEEGKYLILKDPNKQ VIRVYSLPDGTFSSDEDEEEEEEEE EEEEEEETTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: EIF3D eukaryotic translation initiation factor 3 subunit D [ Homo sapiens (human) ]
• Official Symbol: EIF3D
• Synonyms: EIF3S7; eIF3-p66; eIF3-zeta
• Gene ID: 8664
• mRNA Refseq: NM_003753.4
• Protein Refseq: NP_003744.1
• MIM: 603915
• UniProt ID: O15371