Recombinant Full Length Human EIF4E Protein, C-Myc/DDK-tagged

• Cat.No.: EIF4E-12HFL
• Product Overview: Recombinant Full Length Human EIF4E Protein, fused to Myc-DDK-tag at C-terminus, was expressed in HEK293T.

Specification

• Description: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Form: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol.
• Molecular Mass: 24.9 kDa
• AA Sequence: MATVEPETTPTPNPPTTEEEKTESN QEVANPEHYIKHPLQNRWALWFFKN DKSKTWQANLRLISKFDTVE DFWALYNHIQLSSNLMPGCDYSLFK DGIEPMWEDEKNKRGGRWLITLNKQ QRRSDLDRFWLETLLCLIGE SFDDYSDDVCGAVVNVRAKGDKIAI WTTECENREAVTHIGRVYKERLGLP PKIVIGYQSHADTATKSGST TKNRFVV TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >0.05 µg/µL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway

Gene Information

• Gene Name: EIF4E eukaryotic translation initiation factor 4E [ Homo sapiens (human) ]
• Official Symbol: EIF4E
• Synonyms: CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
• Gene ID: 1977
• mRNA Refseq: NM_001968.5
• Protein Refseq: NP_001959.1
• MIM: 133440
• UniProt ID: P06730