Recombinant Full Length Human FLT1 Protein, C-Flag-tagged

• Cat.No.: FLT1-1576HFL
• Product Overview: Recombinant Full Length Human FLT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 148 kDa
• AA Sequence: MVSYWDTGVLLCALLSCLLLTGSSS GSKLKDPELSLKGTQHIMQAGQTLH LQCRGEAAHKWSLPEMVSKE SERLSITKSACGRNGKQFCSTLTLN TAQANHTGFYSCKYLAVPTSKKKET ESAIYIFISDTGRPFVEMYS EIPEIIHMTEGRELVIPCRVTSPNI TVTLKKFPLDTLIPDGKRIIWDSRK GFIISNATYKEIGLLTCEAT VNGHLYKTNYLTHRQTNTIIDVQIS TPRPVKLLRGHTLVLNCTATTPLNT RVQMTWSYPDEKNKRASVRR RIDQSNSHANIFYSVLTIDKMQNKD KGLYTCRVRSGPSFKSVNTSVHIYD KAFITVKHRKQQVLETVAGK RSYRLSMKVKAFPSPEVVWLKDGLP ATEKSARYLTRGYSLIIKDVTEEDA GNYTILLSIKQSNVFKNLTA TLIVNVKPQIYEKAVSSFPDPALYP LGSRQILTCTAYGIPQPTIKWFWHP CNHNHSEARCDFCSNNEESF ILDADSNMGNRIESITQRMAIIEGK NKMASTLVVADSRISGIYICIASNK VGTVGRNISFYITDVPNGFH VNLEKMPTEGEDLKLSCTVNKFLYR DVTWILLRTVNNRTMHYSISKQKMA ITKEHSITLNLTIMNVSLQD SGTYACRARNVYTGEEILQKKEITI RDQEAPYLLRNLSDHTVAISSSTTL DCHANGVPEPQITWFKNNHK IQQEPGIILGPGSSTLFIERVTEED EGVYHCKATNQKGSVESSAYLTVQG TSDKSNLELITLTCTCVAAT LFWLLLTLFIRKMKRSSSEIKTDYL SIIMDPDEVPLDEQCERLPYDASKW EFARERLKLGKSLGRGAFGK VVQASAFGIKKSPTCRTVAVKMLKE GATASEYKALMTELKILTHIGHHLN VVNLLGACTKQGGPLMVIVE YCKYGNLSNYLKSKRDLFFLNKDAA LHMEPKKEKMEPGLEQGKKPRLDSV TSSESFASSGFQEDKSLSDV EEEEDSDGFYKEPITMEDLISYSFQ VARGMEFLSSRKCIHRDLAARNILL SENNVVKICDFGLARDIYKN PDYVRKGDTRLPLKWMAPESIFDKI YSTKSDVWSYGVLLWEIFSLGGSPY PGVQMDEDFCSRLREGMRMR APEYSTPEIYQIMLDCWHRDPKERP RFAELVEKLGDLLQANVQQDGKDYI PINAILTGNSGFTYSTPAFS EDFFKESISAPKFNSGSSDDVRYVN AFKFMSLERIKTFEELLPNATSMFD DYQGDSSTLLASPMLKRFTW TDSKPKASLKIDLRVTSKSKESGLS DVSRPSFCHSSCGHVSEGKRRFTYD HAELERKIACCSPPPDYNSV
  VLYSTPPITRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion

Gene Information

• Gene Name: FLT1 fms related receptor tyrosine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: FLT1
• Synonyms: FLT; FLT-1; VEGFR1; VEGFR-1
• Gene ID: 2321
• mRNA Refseq: NM_002019.4
• Protein Refseq: NP_002010.2
• MIM: 165070
• UniProt ID: P17948

Reviews

07/08/2019

the FLT1 protein is an excellent choice for researchers due to its high quality, reliability, and the comprehensive technical support provided by the manufacturer.

07/17/2018

The manufacturer of FLT1 protein provides excellent technical support, ensuring that researchers receive prompt assistance and guidance when encountering any challenges or difficulties.

05/27/2016

Researchers can confidently incorporate FLT1 protein into their experiments, knowing that they have access to a reliable resource that will meet their experimental needs effectively.

Q&As

How is FLT1 linked to preeclampsia in pregnancy?

Elevated levels of soluble FLT1 have been associated with preeclampsia, a pregnancy complication. Soluble FLT1 acts as a decoy receptor, reducing the bioavailability of VEGF and contributing to vascular dysfunction.

How does FLT1 contribute to retinal diseases such as diabetic retinopathy?

FLT1 has been implicated in retinal diseases like diabetic retinopathy, where abnormal blood vessel growth can lead to vision impairment. Inhibiting FLT1 may help regulate angiogenesis in such conditions.

How is FLT1 expression regulated in the human body?

FLT1 expression is regulated by various factors, including VEGF itself, as well as hypoxia and inflammation. These factors influence the balance of angiogenic signaling.

Can FLT1 be a biomarker for certain diseases?

Yes, elevated levels of soluble FLT1 have been investigated as potential biomarkers for conditions like preeclampsia, cancer, and certain cardiovascular diseases.

Can FLT1 be targeted for anti-angiogenic therapy?

Yes, targeting FLT1 is a strategy for anti-angiogenic therapy. Inhibiting FLT1 can impede blood vessel formation, limiting the blood supply to tumors and potentially slowing their growth.