Recombinant Full Length Human FOLH1 Protein, C-Flag-tagged

• Cat.No.: FOLH1-187HFL
• Product Overview: Recombinant Full Length Human FOLH1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 84.2 kDa
• AA Sequence: MWNLLHETDSAVATARRPRWLCAGA LVLAGGFFLLGFLFGWFIKSSNEAT NITPKHNMKAFLDELKAENI KKFLYNFTQIPHLAGTEQNFQLAKQ IQSQWKEFGLDSVELAHYDVLLSYP NKTHPNYISIINEDGNEIFN TSLFEPPPPGYENVSDIVPPFSAFS PQGMPEGDLVYVNYARTEDFFKLER DMKINCSGKIVIARYGKVFR GNKVKNAQLAGAKGVILYSDPADYF APGVKSYPDGWNLPGGGVQRGNILN LNGAGDPLTPGYPANEYAYR RGIAEAVGLPSIPVHPIGYYDAQKL LEKMGGSAPPDSSWRGSLKVPYNVG PGFTGNFSTQKVKMHIHSTN EVTRIYNVIGTLRGAVEPDRYVILG GHRDSWVFGGIDPQSGAAVVHEIVR SFGTLKKEGWRPRRTILFAS WDAEEFGLLGSTEWAEENSRLLQER GVAYINADSSIEGNYTLRVDCTPLM YSLVHNLTKELKSPDEGFEG KSLYESWTKKSPSPEFSGMPRISKL GSGNDFEVFFQRLGIASGRARYTKN WETNKFSGYPLYHSVYETYE LVEKFYDPMFKYHLTVAQVRGGMVF ELANSIVLPFDCRDYAVVLRKYADK IYSISMKHPQEMKTYSVSFD SLFSAVKNFTEIASKFSERLQDFDK SNPIVLRMMNDQLMFLERAFIDPLG LPDRPFYRHVIYAPSSHNKY
  AGESFPGIYDALFDIESKVDPSKAW GEVKRQIYVAAFTVQAAAETLSEVA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease, Transmembrane

Gene Information

• Gene Name: FOLH1 folate hydrolase 1 [ Homo sapiens (human) ]
• Official Symbol: FOLH1
• Synonyms: PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1
• Gene ID: 2346
• mRNA Refseq: NM_004476.3
• Protein Refseq: NP_004467.1
• MIM: 600934
• UniProt ID: Q04609