Recombinant Full Length Human GARS1 Protein, C-Flag-tagged

• Cat.No.: GARS1-1672HFL
• Product Overview: Recombinant Full Length Human GARS1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 83 kDa
• AA Sequence: MPSPRPVLLRGARAALLLLLPPRLL ARPSLLLRRSLSAASCAPISLPAAA SRSSMDGAGAEEVLAPLRLA VRQQGDLVRKLKEDKAPQVDVDKAV AELKARKRVLEAKELALQPKDDIVD RAKMEDTLKRRFFYDQAFAI YGGVSGLYDFGPVGCALKNNIIQTW RQHFIQEEQILEIDCTMLTPEPVLK TSGHVDKFADFMVKDVKNGE CFRADHLLKAHLQKLMSDKKCSVEK KSEMESVLAQLDNYGQQELADLFVN YNVKSPITGNDLSPPVSFNL MFKTFIGPGGNMPGYLRPETAQGIF LNFKRLLEFNQGKLPFAAAQIGNSF RNEISPRSGLIRVREFTMAE IEHFVDPSEKDHPKFQNVADLHLYL YSAKAQVSGQSARKMRLGDAVEQGV INNTVLGYFIGRIYLYLTKV GISPDKLRFRQHMENEMAHYACDCW DAESKTSYGWIEIVGCADRSCYDLS CHARATKVPLVAEKPLKEPK TVNVVQFEPSKGAIGKAYKKDAKLV MEYLAICDECYITEMEMLLNEKGEF TIETEGKTFQLTKDMINVKR FQKTLYVEEVVPNVIEPSFGLGRIM YTVFEHTFHVREGDEQRTFFSFPAV VAPFKCSVLPLSQNQEFMPF VKELSEALTRHGVSHKVDDSSGSIG RRYARTDEIGVAFGVTIDFDTVNKT PHTATLRDRDSMRQIRAEIS
  ELPSIVQDLANGNITWADVEARYPL FEGQETGKKETIEETRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Aminoacyl-tRNA biosynthesis

Gene Information

• Gene Name: GARS1 glycyl-tRNA synthetase 1 [ Homo sapiens (human) ]
• Official Symbol: GARS1
• Synonyms: GARS; HMN5; CMT2D; DSMAV; GlyRS; HMN5A; SMAD1; SMAJI
• Gene ID: 2617
• mRNA Refseq: NM_002047.4
• Protein Refseq: NP_002038.2
• MIM: 600287
• UniProt ID: P41250