Recombinant Full Length Human HARS2 Protein, C-Flag-tagged

• Cat.No.: HARS2-2177HFL
• Product Overview: Recombinant Full Length Human HARS2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 53.3 kDa
• AA Sequence: MPLLGLLPRRAWASLLSQLLRPPCA SCTGAVRCQSQVAEAVLTSQLKAHQ EKPNFIIKTPKGTRDLSPQH MVVREKILDLVISCFKRHGAKGMDT PAFELKETLTEKYGEDSGLMYDLKD QGGELLSLRYDLTVPFARYL AMNKVKKMKRYHVGKVWRRESPTIV QGRYREFCQCDFDIAGQFDPMIPDA ECLKIMCEILSGLQLGDFLI KVNDRRIVDGMFAVCGVPESKFRAI CSSIDKLDKMAWKDVRHEMVVKKGL APEVADRIGDYVQCHGGVSL VEQMFQDPRLSQNKQALEGLGDLKL LFEYLTLFGIADKISFDLSLARGLD YYTGVIYEAVLLQTPTQAGE EPLNVGSVAAGGRYDGLVGMFDPKG HKVPCVGLSIGVERIFYIVEQRMKT KGEKVRTTETQVFVATPQKN FLQERLKLIAELWDSGIKAEMLYKN NPKLLTQLHYCESTGIPLVVIIGEQ ELKEGVIKIRSVASREEVAI KRENFVAEIQKRLSES TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Aminoacyl-tRNA biosynthesis

Gene Information

• Gene Name: HARS2 histidyl-tRNA synthetase 2, mitochondrial [ Homo sapiens (human) ]
• Official Symbol: HARS2
• Synonyms: HO3; HARSL; HARSR; HisRS; PRLTS2
• Gene ID: 23438
• mRNA Refseq: NM_012208.4
• Protein Refseq: NP_036340.1
• MIM: 600783
• UniProt ID: P49590