Recombinant Full Length Human HES1 Protein, C-Flag-tagged

• Cat.No.: HES1-1831HFL
• Product Overview: Recombinant Full Length Human HES1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 29.4 kDa
• AA Sequence: MPADIMEKNSSSPVAATPASVNTTP DKPKTASEHRKSSKPIMEKRRRARI NESLSQLKTLILDALKKDSS RHSKLEKADILEMTVKHLRNLQRAQ MTAALSTDPSVLGKYRAGFSECMNE VTRFLSTCEGVNTEVRTRLL GHLANCMTQINAMTYPGQPHPALQA PPPPPPGPGGPQHAPFAPPPPLVPI PGGAAPPPGGAPCKLGSQAG EAAKVFGGFQVVPAPDGQFAFLIPN GAFAHSGPVIPVYTSNSGTSVGPNA VSPSSGPSLTADSMWRPWRN TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young, Notch signaling pathway

Gene Information

• Gene Name: HES1 hes family bHLH transcription factor 1 [ Homo sapiens (human) ]
• Official Symbol: HES1
• Synonyms: HHL; HRY; HES-1; bHLHb39
• Gene ID: 3280
• mRNA Refseq: NM_005524.4
• Protein Refseq: NP_005515.1
• MIM: 139605
• UniProt ID: Q14469