Recombinant Full Length Human HEXB Protein, C-Flag-tagged

• Cat.No.: HEXB-1983HFL
• Product Overview: Recombinant Full Length Human HEXB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 62.9 kDa
• AA Sequence: MELCGLGLPRPPMLLALLLATLLAA MLALLTQVALVVQVAEAARAPSVSA KPGPALWPLPLSVKMTPNLL HLAPENFYISHSPNSTAGPSCTLLE EAFRRYHGYIFGFYKWHHEPAEFQA KTQVQQLLVSITLQSECDAF PNISSDESYTLLVKEPVAVLKANRV WGALRGLETFSQLVYQDSYGTFTIN ESTIIDSPRFSHRGILIDTS RHYLPVKIILKTLDAMAFNKFNVLH WHIVDDQSFPYQSITFPELSNKGSY SLSHVYTPNDVRMVIEYARL RGIRVLPEFDTPGHTLSWGKGQKDL LTPCYSRQNKLDSFGPINPTLNTTY SFLTTFFKEISEVFPDQFIH LGGDEVEFKCWESNPKIQDFMRQKG FGTDFKKLESFYIQKVLDIIATINK GSIVWQEVFDDKAKLAPGTI VEVWKDSAYPEELSRVTASGFPVIL SAPWYLDLISYGQDWRKYYKVEPLD FGGTQKQKQLFIGGEACLWG EYVDATNLTPRLWPRASAVGERLWS SKDVRDMDDAYDRLTRHRCRMVERG IAAQPLYAGYCNHENM TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Lysosome, Metabolic pathways, Other glycan degradation

Gene Information

• Gene Name: HEXB hexosaminidase subunit beta [ Homo sapiens (human) ]
• Official Symbol: HEXB
• Synonyms: ENC-1AS, HEL-248, HEL-S-111
• Gene ID: 3074
• mRNA Refseq: NM_000521.4
• Protein Refseq: NP_000512.2
• MIM: 606873
• UniProt ID: P07686