Recombinant Full Length Human HLA-E Protein, C-Flag-tagged

• Cat.No.: HLA-E-268HFL
• Product Overview: Recombinant Full Length Human HLA-E Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.8 kDa
• AA Sequence: MVDGTLLLLLSEALALTQTWAGSHS LKYFHTSVSRPGRGEPRFISVGYVD DTQFVRFDNDAASPRMVPRA PWMEQEGSEYWDRETRSARDTAQIF RVNLRTLRGYYNQSEAGSHTLQWMH GCELGPDGRFLRGYEQFAYD GKDYLTLNEDLRSWTAVDTAAQISE QKSNDASEAEHQRAYLEDTCVEWLH KYLEKGKETLLHLEPPKTHV THHPISDHEATLRCWALGFYPAEIT LTWQQDGEGHTQDTELVETRPAGDG TFQKWAAVVVPSGEEQRYTC HVQHEGLPEPVTLRWKPASQPTIPI VGIIAGLVLLGSVVSGAVVAAVIWR KKSSGGKGGSYSKAEWSDSA
  QGSESHSLTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis

Gene Information

• Gene Name: HLA-E major histocompatibility complex, class I, E [ Homo sapiens (human) ]
• Official Symbol: HLA-E
• Synonyms: QA1; HLA-6.2
• Gene ID: 3133
• mRNA Refseq: NM_005516.6
• Protein Refseq: NP_005507.3
• MIM: 143010
• UniProt ID: P13747