Recombinant Full Length Human HSPA15 Protein, C-Flag-tagged

• Cat.No.: ITGA8-1313HFL
• Product Overview: Recombinant Full Length Human ITGA8 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 117.3 kDa
• AA Sequence: MSPGASRGPRGSQAPLIAPLCCAAA ALGMLLWSPACQAFNLDVEKLTVYS GPKGSYFGYAVDFHIPDART ASVLVGAPKANTSQPDIVEGGAVYY CPWPAEGSAQCRQIPFDTTNNRKIR VNGTKEPIEFKSNQWFGATV KAHKGKVVACAPLYHWRTLKPTPEK DPVGTCYVAIQNFSAYAEFSPCRNS NADPEGQGYCQAGFSLDFYK NGDLIVGGPGSFYWQGQVITASVAD IIANYSFKDILRKLAGEKQTEVAPA SYDDSYLGYSVAAGEFTGDS QQELVAGIPRGAQNFGYVSIINSTD MTFIQNFTGEQMASYFGYTVVVSDV NSDGLDDVLVGAPLFMEREF ESNPREVGQIYLYLQVSSLLFRDPQ ILTGTETFGRFGSAMAHLGDLNQDG YNDIAIGVPFAGKDQRGKVL IYNGNKDGLNTKPSQVLQGVWASHA VPSGFGFTLRGDSDIDKNDYPDLIV GAFGTGKVAVYRARPVVTVD AQLLLHPMIINLENKTCQVPDSMTS AACFSLRVCASVTGQSIANTIVLMA EVQLDSLKQKGAIKRTLFLD NHQAHRVFPLVIKRQKSHQCQDFIV YLRDETEFRDKLSPINISLNYSLDE STFKEGLEVKPILNYYRENI VSEQAHILVDCGEDNLCVPDLKLSA RPDKHQVIIGDENHLMLIINARNEG EGAYEAELFVMIPEEADYVG IERNNKGFRPLSCEYKMENVTRMVV CDLGNPMVSGTNYSLGLRFAVPRLE KTNMSINFDLQIRSSNKDNP DSNFVSLQINITAVAQVEIRGVSHP PQIVLPIHNWEPEEEPHKEEEVGPL VEHIYELHNIGPSTISDTIL EVGWPFSARDEFLLYIFHIQTLGPL QCQPNPNINPQDIKPAASPEDTPEL SAFLRNSTIPHLVRKRDVHV VEFHRQSPAKILNCTNIECLQISCA VGRLEGGESAVLKVRSRLWAHTFLQ RKNDPYALASLVSFEVKKMP YTDQPAKLPEGSIAIKTSVIWATPN VSFSIPLWVIILAILLGLLVLAILT LALWKCGFFDRARPPQEDMT
  DREQLTNDKTPEATRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton

Gene Information

• Gene Name: ITGA8 integrin subunit alpha 8 [ Homo sapiens (human) ]
• Official Symbol: ITGA8
• Synonyms: integrin, alpha 8; OTTHUMP00000019207
• Gene ID: 8516
• mRNA Refseq: NM_003638.3
• Protein Refseq: NP_003629.2
• MIM: 604063
• UniProt ID: P53708