Recombinant Full Length Human IDH1 Protein, C-Flag-tagged

• Cat.No.: IDH1-1116HFL
• Product Overview: Recombinant Full Length Human IDH1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.5 kDa
• AA Sequence: MSKKISGGSVVEMQGDEMTRIIWEL IKEKLIFPYVELDLHSYDLGIENRD ATNDQVTKDAAEAIKKHNVG VKCATITPDEKRVEEFKLKQMWKSP NGTIRNILGGTVFREAIICKNIPRL VSGWVKPIIIGRHAYGDQYR ATDFVVPGPGKVEITYTPSDGTQKV TYLVHNFEEGGGVAMGMYNQDKSIE DFAHSSFQMALSKGWPLYLS TKNTILKKYDGRFKDIFQEIYDKQY KSQFEAQKIWYEHRLIDDMVAQAMK SEGGFIWACKNYDGDVQSDS VAQGYGSLGMMTSVLVCPDGKTVEA ESAHGTVTRHYRMYQKGQETSTNPI ASIFAWTRGLAHRAKLDNNK
  ELAFFANALEEVSIETIEAGFMTKD LAACIKGLPNVQRSDYLNTFEFMDK LGENLKIKLAQAKLTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

Gene Information

• Gene Name: IDH1 isocitrate dehydrogenase (NADP(+)) 1 [ Homo sapiens (human) ]
• Official Symbol: IDH1
• Synonyms: IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• Gene ID: 3417
• mRNA Refseq: NM_005896.4
• Protein Refseq: NP_005887.2
• MIM: 147700
• UniProt ID: O75874