Recombinant Full Length Human IGF1R Protein, C-Flag-tagged

• Cat.No.: IGF1R-453HFL
• Product Overview: Recombinant Full Length Human IGF1R Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 81 kDa
• AA Sequence: MKSGSGGGSPTSLWGLLFLSAALSL WPTSGEICGPGIDIRNDYQQLKRLE NCTVIEGYLHILLISKAEDY RSYRFPKLTVITEYLLLFRVAGLES LGDLFPNLTVIRGWKLFYNYALVIF EMTNLKDIGLYNLRNITRGA IRIEKNADLCYLSTVDWSLILDAVS NNYIVGNKPPKECGDLCPGTMEEKP MCEKTTINNEYNYRCWTTNR CQKMCPSTCGKRACTENNECCHPEC LGSCSAPDNDTACVACRHYYYAGVC VPACPPNTYRFEGWRCVDRD FCANILSAESSDSEGFVIHDGECMQ ECPSGFIRNGSQSMYCIPCEGPCPK VCEEEKKTKTIDSVTSAQML QGCTIFKGNLLINIRRGNNIASELE NFMGLIEVVTGYVKIRHSHALVSLS FLKNLRLILGEEQLEGNYSF YVLDNQNLQQLWDWDHRNLTIKAGK MYFAFNPKLCVSEIYRMEEVTGTKG RQSKGDINTRNNGERASCES DVLHFTSTTTSKNRIIITWHRYRPP DYRDLISFTVYYKEAPFKNVTEYDG QDACGSNSWNMVDVDLPPNK DVEPGILLHGLKPWTQYAVYVKAVT LTMVENDHIRGAKSEILYIRTNASV PSIPLDVLSASNSSSQLIVK WNPPSLPNGNLSYYIVRWQRQPQDG YLYRHNYCSKDKIPIRKYADGTIDI EEVTENPKTEVCGGEKGPCC ACPKTEAEKQAEKEEAEYRKVFENF LHNSIFVPRPERKRRDVMQVANTTM SSRSRNTTAADTYNITDPEE LETEYPFFESRVDNKERTVISNLRP FTLYRIDIHSCNHEAEKLGCSASNF VFARTMPAEGADDIPGPVTW EPRPENSIFLKWPEPENPNGLILMY EIKYGSQVEDQRECVSRQEYRKYGG AKLNRLNPGNYTARIQATSL SGNGSWTDPVFFYVQAKTGYENFIH LIIALPVAVLLIVGGLVIMLYVFHR KRNNSRLGNGVLYASVNPEY FSAADVYVPDEWEVAREKITMSREL GQGSFGMVYEGVAKGVVKDEPETRV AIKTVNEAASMRERIEFLNE ASVMKEFNCHHVVRLLGVVSQGQPT LVIMELMTRGDLKSYLRSLRPEMEN NPVLAPPSLSKMIQMAGEIA DGMAYLNANKFVHRDLAARNCMVAE DFTVKIGDFGMTRDIYETDYYRKGG KGLLPVRWMSPESLKDGVFT TYSDVWSFGVVLWEIATLAEQPYQG LSNEQVLRFVMEGGLLDKPDNCPDM LFELMRMCWQYNPKMRPSFL EIISSIKEEMEPGFREVSFYYSEEN KLPEPEELDLEPENMESVPLDPSAS SSSLPLPDRHSGHKAENGPG
  PGVLVLRASFDERQPYAHMNGGRKN ERALPLPQSSTCTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Colorectal cancer, Endocytosis, Focal adhesion, Glioma, Long-term depression, Melanoma, Oocyte meiosis, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer

Gene Information

• Gene Name: IGF1R insulin like growth factor 1 receptor [ Homo sapiens (human) ]
• Official Symbol: IGF1R
• Synonyms: IGFR; CD221; IGFIR; JTK13
• Gene ID: 3480
• mRNA Refseq: NM_000875.5
• Protein Refseq: NP_000866.1
• MIM: 147370
• UniProt ID: P08069

Reviews

07/08/2022

The product's exceptional purity facilitated precise quantification, ensuring data consistency.

06/14/2019

The product consistently delivered robust and dependable results, affirming its efficacy in our experiments.

04/29/2018

The product's consistent performance bolstered the dependability of our research conclusions.

Q&As

In which cellular compartments is IGF1R predominantly localized?

IGF1R is predominantly localized on the cell membrane, where it acts as a receptor for insulin-like growth factors (IGFs) and insulin.

Are there specific downstream signaling pathways activated by IGF1R, and how do they influence cell proliferation and survival?

Downstream signaling pathways activated by IGF1R include the Ras/MAPK pathway and PI3K/Akt pathway, contributing to cell growth and survival.

How does IGF1R interact with insulin-like growth factors (IGFs) and insulin, and what is its role in growth regulation?

IGF1R interacts with IGFs and insulin, triggering downstream signaling pathways such as the PI3K/Akt pathway, influencing cell proliferation and survival.

Are there therapeutic implications or potential targets related to IGF1R in diseases involving growth signaling?

There are potential therapeutic implications related to IGF1R in diseases involving growth signaling, making it a target for drug development.

What diseases or conditions are associated with dysregulation or abnormal expression of IGF1R?

Dysregulation or abnormal expression of IGF1R is associated with conditions such as cancer and developmental disorders.

What is the primary function of Insulin-like growth factor 1 receptor (IGF1R) in cellular processes and growth signaling?

Insulin-like growth factor 1 receptor (IGF1R) functions in cellular processes, primarily playing a role in growth signaling.

How does IGF1R contribute to the regulation of tissue development, particularly in the context of growth and differentiation?

IGF1R contributes to the regulation of tissue development by influencing cell growth, differentiation, and survival.